Ideally, all these SNPs should be replicated in a large and independent series of cases and controls to further confirm their involvement in DTC predisposition SNPs within cell-cycle regulation and apoptosis genes Thirty-three common SNPs in 15 genes involved in the cell-cycle regulation or in apoptosis were collected.
Disclosure of Potential Conflicts of Interest No potential conflicts of interest were disclosed. Thus, no significant evidence of association was identified in the meta-analysis using the random-effect model Table 2. SNPs previously studied in relation to DTC risks from genome-wide association studies or studies focused on specific intergenic regions Genetic variants on 1p In order to gain further insights into the role of SNPs previously associated with DTC, in the present work we carefully analyzed the results of our GWAS and we performed meta-analyses with the previous studies.
In particular, we highlighted the role of rs and rs, whose association was not significant in previous studies but became statistically significant after increasing the sample size with the present meta-analysis. The role of these loci in DTC etiology was already discussed in previous works and will be not discussed here.
This article must therefore be hereby marked advertisement in accordance with 18 U.
Landi Administrative, technical, or material support i. Landi Development of methodology: Of them, 10 were associated with the disease in the literature with at least one genetic model but none was replicated in the present GWAS under the allelic model Table 1.
Furthermore, rs near NKX 14q Accepted January 23, Overall, 19 positive associations were reported in the literature.
Further cutting-edge studies, as novel GWASs, next-generation sequencing analysis, fine-mapping or genome-wide interactions studies, are needed to characterize all the predisposing risk factors for DTC.
When the meta-analyses were extended to other available populations, four more SNPs showed an evidence of association, although not significantly after FDR correction: The costs of publication of this article were defrayed in part by the payment of page charges. Thus, the meta-analyses did not confirm the role of these variants in DTC etiology Tables 1 and 2.
Received June 29, Section solely to indicate this fact. Furthermore, to date, only few GWASs were performed, and a small number of genomic loci were associated with the risk of the disease by using this approach. SNPs within immune response and inflammation genes Fifteen genes and 33 SNPs involved in immunity or in inflammation pathways were analyzed to identify susceptibility variants for DTC and eight significantly associated SNPs were published Table 1.
Moreover, the present meta-analysis points rs 1p11 THEDYNAMICPROBLEM Uptothispoint,nosimplifyingassumptionshavebeenused. Inthefoixeanalysis,however,itmustbeassiunedthatthelinks behaveasperfectlyrigidbodies. Methods: We combined the results from a genome-wide association study (GWAS) performed by our group and from published studies on DTC.
With a first approach, we evaluated whether a SNP published as associated with the risk of DTC could replicate in our GWAS (using FDR as adjustment for multiple comparisons).
A Comprehensive Meta-analysis of.
METHODS OF COLLECTING AND ANALYZING EMPIRICAL MATERIALSData Management and Analysis Methods 29 DATA MANAGEMENT AND ANALYSIS METHODS u. SAMURAI: Sensitivity analysis of a meta-analysis with unpublished but registered analytical investigations (software). methods/technologies in the quality control of herbal medicinal products1F EMA/HMPC// Page 4/4 European Pharmacopoeia General Chapter 2 “Methods o f analysis”.
Evolving CMC Analytical Techniques for Biopharmaceuticals. 21 October, Abstract During the (early) preclinical drug development process as well as manufacturing of biopharmaceutical (protein) products, analysis and characterization are crucial in gaining a better understand.Download